Genome Editing With Engineered Zinc Finger Nucleases

Genome Editing With Engineered Zinc Finger Nucleases. The most commonly used genomeediting tools are zincfinger nucleases (ZFNs) transcription activatorlike effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)CRISPRassociated 9 (Cas9) 32 Each represents a type of engineered nuclease that can be customized to recognize bind and cleave a specific sequence in the genome.

Here we report rapid and targeted genetic engineering of this parasite using zincfinger nucleases (ZFNs) that produce a doublestrand break in a userdefined locus and trigger homologydirected repair Targeting an integrated egfp locus we obtained genedeletion parasites with unprecedented speed (2 weeks) both with and without direct selection.

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OverviewDomainsApplicationsPotential problemsProspectsZincfinger nickasesZincfinger nucleases are artificial restriction enzymes generated by fusing a zinc finger DNAbinding domain to a DNAcleavage domain Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zincfinger nucleases to target unique sequences within complex genomes By taking advantage of endogenous DNA repair machiner Text under.

Altmetric – Genome editing with engineered zinc finger

There are three principal classes of engineered nucleases Zinc finger nucleases (ZFNs) consist of a DNAbinding domain derived from zincfinger proteins linked to.

Genome Engineering With ZincFinger Nucleases …

Zincfinger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as genetargeting tools ZFNinduced doublestrand breaks are subject to cellular DNA repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies.